果蠅實驗指南

果蠅實驗指南

图书基本信息
出版时间:2003-12
出版社:科學出版社
作者:W.沙利文 編
页数:697
字数:1564000
书名:果蠅實驗指南
封面图片
果蠅實驗指南

内容概要
果蠅是遺傳學、發育學研究的重要模式生物之一,對于發育生物學、基因組學領域中的探索具有極為重要的意義。本書是冷臬港實驗室出版社Drosophila Protocols的影印版,共提供了近40個未來10年內最可能用到的以果蠅為實驗對象的分子生物學、生物化學和細胞生物學研究的實驗方案。每一個方案都經過專家的精心挑選和雕琢,實驗設計嚴謹、準確、簡潔、規範,可操作性強,值得稱道。方案涉及染色體、細胞、基因組和發育等方面,既包含了初學者需要了解的基礎知識,也涵蓋了資深研究者所需的細節。本書的版式設計側重于方便讀者使用,正文中穿插了豐富的圖表作為實驗設計的輔助說明,附錄中還列出了果蠅研究所需的重要資料如基本溶液、緩沖液的配方和配制方法等。    本書適合于從事發育生物學、細胞生物學、基因組學、遺傳學、分子生物學、藥物設計和開發以及功能基因學組研究的相關教學科研人員以及相關學科的本科生、研究生參考使用。
书籍目录
PrefaceAbbreviationsCHROMOSOMES 1 Preparation and Analysis of Drosophila Mitotic Chromosomes  2 In Situ Hybridization to Somatic Chromosomes  3 BrdU Labeling of Chromosomes  4 Analysis of Meiosis of Fixed and Live Oocytes by Light Microscopy  5 Cytological Analysis of Spermatocyte Growth and Male Meiosis in Drosophila melanogaster  6 Preparation and Analysis of Polytene Chromosomes  7 In Situ Hybridizaiton to Polytene Chromosomes  8 Mapping Protein Distributions on Polytene Chromosomes by ImmunostainingCELL BIOLOGY  9 Fluorescent Analysis of Drosophila Embryos  10 Imaginal Discs  11 The Neuromuscular Junction  12 Histological Techniques for the Drosophila Eye.Part Ⅰ:Larva and Pupa  13 Histological Techniques for the Drosophila Eye.Part Ⅱ:Adult  14 Preparation of Thin Sections of Drosophila for Examination by Transmission Electron Microscopy  15 Electrophysiological Approaches to the Neuromusculature  16 Functional Assays of the Peripheral and Central Nervous Systems  17 GFP and Other Reporters  18 Quantitative Microinjection of Drosophila Embryos  19 Targeted Disruption of Gene Function in Drosophila by RNA Interference  20 Drosophila Cell Culture and TRransformation  21 Generating Antibodies against Drosophila Proteins  22 Photoactivated Gene Expression for Cell-fate Mapping and Cell ManipulationMOLECULAR BIOLOGY  23 Recovery of DNA Sequences Flanking P-element Insertions:Inverse PCR and Plasmid Rescue  24 GAL4-mediated Ectopic Gene Expression in Drosophila  25 Functional Cell AblationGENOMICS  26 Preparation of DNA from E.coli Cells Containing Bacteriophage P1 Clones  27 Drosophila Microarrays:From Arrayer Construction to Hybridization  28 Using Drosophila Genome Databases  29 Preparation and Analysis of High-molecular-weight DNA in DrosophilaBIOCHEMISTRY  30 Culturing Large Populations of Drosophila for Protein Biochemistry  31 Preparation of Nuclear Extracts from Drosophila Embryos and In Vitro Transcription Analysis  32 Preparation of Membrane Proteins from Drosophila Embryos  33 Preparing Cytoplasmic Extracts from Drosophila Embryos  34 Immunoblotting of Proteins from Single Drosophila EmbryosTHE ORGANISM  35 Laboratory Culture of Drosophila  36 Preparation of Larval and Adult Cuticles for Light Microscopy  37 Exposing Drosophila to Neuroactive DrugsAPPENDICES  1 1989 Table of Contents from Drosophila:A Laboratory Manual  2 Anatomical Drawings of Drosophila  3 Solutions and Reagents  4 Cautions  5 Suppliers  6 TrademarksIndex



下载链接

果蠅實驗指南下載

评论与打分
  •     購貨和發票應該一起發送給客戶。這樣免得客戶再索要發票,增加手續。當然,我知道你們可能想少開發票,增加收入。